全國(guó)統(tǒng)一(yī)銷售(shòu)熱(rè)綫：
全國(guó)統(tǒng)一(yī)銷售(shòu)熱(rè)綫：活體轉基(jī)因(yīn)--鉗(qián)狀電極用於體(tǐ)內(nèi)藥物/基因(yīn)/蛋白(bái)質傳(chuán)遞及基因(yīn)治(zhì)療．--鉗(qián)狀電極Tweezertrodes電極(jí)是一種(zhǒng)可復用(yòng)的(dí)小(xiǎo)鉗(qián)狀(zhuàng)電(diàn)極，主要用(yòng)於體(tǐ)內的藥(yào)物(wù)或(huò)基(jī)因(yīn)的(dí)傳遞。--鉗狀(zhuàng)電極(jí)是一(yī)個標準的11.5厘米的(dí)小(xiǎo)鉗子(zǐ)，其(qí)尖(jiān)部(bù)為(wéi)嵌(qiàn)入的不鏽(xiù)鋼圓(yuán)盤狀電(diàn)極，兩盤(pán)的間隙(xì)可調至2厘(lí)米，並(bìng)有一個陽(yáng)極指示器(qì)。
活(huó)體(tǐ)轉基因(yīn)--鉗(qián)狀電極(jí)用於體(tǐ)內(nèi)藥物(wù)/基(jī)因/蛋白質傳(chuán)遞及(jí)基因(yīn)治療電極．
活(huó)體轉基因--鉗狀電(diàn)極Tweezertrodes是(shì)一種可(kě)復用(yòng)的(dí)小鉗狀(zhuàng)電(diàn)極，主要用於體內的藥物或(huò)基因的(dí)傳遞(dì)。電極是(shì)一個(gè)標(biāo)準的(dí)11.5厘米的(dí)小鉗子，其尖(jiān)部為(wéi)嵌入的不(bù)鏽鋼(gāng)圓盤狀電(diàn)極(jí)，兩盤的間隙(xì)可調至(zhì)2厘米，並有(yǒu)一(yī)個陽(yáng)極指示器(qì)。
Tweezertrodes電極(jí)有兩(liǎng)種規(guī)格(gé)，45-0118為(wéi)直徑：7mm的電極盤(pán)，45-0119為(wéi)直徑：10mm的(dí)電極盤
當(dāng)把感(gǎn)興(xīng)趣的分子注(zhù)射到(dào)體(tǐ)內(nèi)後，用(yòng)電極(jí)盤夾住相(xiāng)關組織，然(rán)後給出電脈(mài)衝(chōng)，使(shǐ)孔道開始形(xíng)成(chéng)，將(jiāng)分子整合到直(zhí)接與(yǔ)電(diàn)極(jí)盤接觸的(dí)組(zǔ)織細胞(bāo)內。
Tweezertrodes are reusable, tweezer-style in vivo electrodes fordrug or gene delivery in animals. Tweezertrodes consist of astandard 11.5 cm tweezer that has been modified with stainlesssteel circular or disk electrodes at the tip. The gap between theelectrode disks may be adjusted from under 1 mm to over 2 cm.Tweezertrodes come in two models. Model 520 has an electrode diskdiameter of 7 mm and Model 522 has a diameter of 10 mm.Tweezertrodes may be cleaned with a mild detergent and sterilizedwith ethanol or ethylene oxide. These electrodes are connected to apulse generator with the Model 524 Connection Cable, and arecompatible with most BTX Electroporation System
Applications
Tweezertrodes may be used for many in vivo applications, includinggene and drug delivery. Following localized or systemic injectionof the molecule of interest, the Tweezertrode electrode disks areused to grasp the tissue of interest. An electroporation pulse isthen given, initiating pore formation and incorporation of themolecule into the cells of the tissue in direct contact with theelectrode disks. Suzuki et al. used Tweezertrodes to deliver aGreen Fluorescent Protein (GFP) gene into rat liver. The authorsdemonstrated successful gene transfer and expression in situ.1,2Saito and Nakatsuji used a similar electrode to introduce plasmidDNA during ex-utero and in-utero electroporation.7 Sato et al. usedthe Tweezertrode with the T820 to introduce EGFP expressing plasmidin mouse testis.8 Tweezertrodes complement BTX Genetrode Models508, 510, Caliper Electrode Models 384 and 384L, 2-Needle ArrayModels 530 and 532 as the newest addition to our in vivo electrodeline. The Tweezertrodes can be used to reproduce applicationsrequiring the caliper electrodes, including gene therapy,transdermal drug delivery and electroporation therapy.3,4,5,6
Technical Specifications
Standard Capabilities
Voltage Range: 0V - 200V (Do not use AC current)
Pulse Length Range: 1 µsec - 200 msec
Physical Characteristics
Model 520
Tweezertrode Length: 11.5 cm
Electrode Diameter: 7 mm
Electrode Material: Stainless steel
Model 522
Tweezertrode Length: 11.5 cm
Electrode Diameter: 10 mm
Electrode Material: Stainless steel
Generator Compatibility ECM T820, 630, 830, 2001
Ordering Information
System Model Available Configurations
520 Tweezertrode 520
Tweezer Electrode with stainless steel plated 7 mm diameterelectrode disk for in vivo applications
523 Tweezertrode 523
Tweezer Electrode with stainless steel plated 10mm diameterelectrode disk for in vivo applications
Accessory Model Description
524 Tweezertrode Connection Cable
References
Suzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivoElectroporation, FEBS Letters, 425: 436-440 (1998)
BTX Electroporation Protocol PR0363 (1998)
Zhang et al., In vivo transdermal delivery of large molecules bypressure-mediated electroincorporation and electroporation: a novelmethod for drug and gene delivery, Bioelectrochemistry andBioenergetics, 42: 283-292 (1997)
Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotidartery by electroporation, Third U.S. / Japan Symposium on DrugDelivery Systems (Abstr.), 38 (1995)
Dev, Killing cancer cells with a combination of pulsed electricfields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)
BTX Electroporation Therapy Protocols ECT001-ECT005 (1995)
Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001)
Sato et al., Molecular Reproduction and Development, 61: 49-56(2002)
